DEVELOPMENT OF AN AUTOMATED IMMUNOTURBIDIMETRIC FERRITIN ASSAY PDF
Such assays are suitable for the measurement of proteins at concentrations >5– 10 mg/L. .. Development of an automated immunoturbidimetric ferritin assay. May 3, Different laboratory methods are used to quantify ferritin . assays and automated equipment developments, and in routine laboratory assays and Seco M. Development of an automated immunoturbidimetric ferritin assay. J. Colloid. Interface Sci. (2): – 3. Borque, L., Rus, A., Bellod, L., and Seco, M.L. Development of an automated immunoturbidimetric ferritin assay.
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The quantitative assessment of body iron. Colloidal particles coated with polyclonal IgG usually are not very stable, mainly because of the high antibody coverage needed to obtain good sensitivities 3. Posttreatment with additives BSA, surfactants has been proposed 13 14although it reduces IgG charge sn reactivity. The turbidimetric method affords a measuring range of 0. Ann Clin Biochem ; Biological or Clinical Significance: CRP is a prognostic risk factor for coronary heart disease in both patients with angina stable or not and in apparently healthy subjects 2and distinguishes rapidly destructive vs slowly progressive osteoarthritis 3.
We have developed a new procedure for turbidimetric measurement of ferritin concentration in human serum, based on latex microparticle agglutination technology. The immunopurified antibody showed a similar trend, having a covalently coupled adsorption isotherm according to that of the nonimmunopurified antibody in the range studied 0.
The ferritin assay is an automated immunoturbidimetric method. Recent studies of CRP concentrations within the conventional reference interval have suggested new clinical applications.
In conclusion, a low coverage 0. Skip to main content. This approach to antibody immunopurification could be extended to obtain reagents useful for the measurement of several other proteins at low concentrations A microparticle size of nm and a wavelength of nm were selected as the best choice because of the high slope and wide dynamic range showed. Ferritin determinations are widely used for monitoring renal anemia when iron utilization and distribution disorders are present during therapy with erythropoietin.
We found that at any given working dilution of developmnet studied, the initial absorbance decreases with increasing wavelength and increases with microparticle diameter, according to the light-scattering theory.
Biomarker Menu Search Search by Keyword: Biochemical markers and hematologic indices in the diagnosis of functional iron deficiency.
View inline View popup. We describe a microparticle reagent that we use in an optimized turbidimetric procedure with immunopurified polyclonal antibodies against CRP. Increased serum C-reactive protein levels by immunonephelometry gerritin patients with rapidly destructive hip osteoarthritis.
Research Article Auutomated Brief. The microparticle reagents were prepared according to a previously published method by the carbodiimide [1-ethyl 3-dimethylamino-propyl carbodiimide chloride] procedure CRP measurements have been used for many years in the management of a variety of clinical situations, such as bacterial infections, ischemic necrosis of tissue, and active inflammatory conditions 1. Eur J Pediatr ; Development and validation of a particle enhanced turbidimetric immunoassay for Mimunoturbidimetric protein.
It is also used to distinguish between hypoferric anemia and hypochromic anemia chronic infection and tumor anemias, sideroblastic anemia or thalassemia. A new method of measuring C-reactive protein, with a low limit of detection, suitable for risk assessment of coronary heart disease.
Development of an automated immunoturbidimetric ferritin assay.
Evaluation of an improved immunonephelometric assay for C-reactive protein [Abstract]. New methods of measuring CRP have lower limits of detection one-tenth those of earlier methods 8 9.
Ceffect of particle size on absorbance at nm. The determination of ferritin is useful in diagnosis of defects in iron metabolism, monitoring iron therapy, ascertaining the iron reserves in groups at risk and in the differential diagnosis of anemias. J Clin Immunoassay ; We suggest an alternative approach that uses a small amount of immunopurified antibody and stabilizes the reagent by saturation of free surface with BSA in a two-step sequential covalent procedure.
The effect of varying the particle size on a reagent with a constant charge of 0. Lancet ; The detection limits for CRP, defined as the mean plus 3 SD of the zero signal saline solutionwere 0. The microparticle reagent response of the different antibodies to increasing CRP concentrations can be seen in Fig.
Rev Rheum Engl Ed ; The procedure has been automated using the Falcor analyzer. Conditions for CRP determination. We monitored the reaction kinetics at pH values of 6. Parameter values abcorrelation coefficients rand confidence regions were as follows: Samples with CRP values above the upper limit of the calibration curve can be reanalyzed after automated or manual sample dilution.
Among the many commercially available assays for CRP, the most common use fluid-phase or particle-enhanced turbidimetric or nephelometric procedures 7. The covalently coupled isotherms of IgG anti-CRP on carboxyl-modified polystyrene particles nm diameter are shown in Fig.
The maximum adsorption plateau is achieved at a polyclonal antibody concentrations of 3. Clinical Chemistry Nov46 11. Development of an automated immunoturbidimetric ferritin assay. After elution, the released protein verritin measured using the BCA procedure.