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Infection by Grapevine fanleaf nepovirus (GFLV), a bipartite RNA virus of positive polarity belonging to the Comoviridae family, causes extensive cytopathic. The specific transmission of Grapevine fanleaf virus by its nematode vector Xiphinema index is solely determined by the viral coat protein. There are plenty of plant viruses that no one has heard of, but few are as widely known as grapevine fanleaf virus. Learn how to identify a sick.

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Consistent with its function in cell-to-cell movement, the movement protein was generally detected at the cell periphery at 48 hpi, probably after its release from the precursor polyprotein. The fact that distinct types of membranes are involved in the replication of different viruses implies the establishment of specific interactions between such host membranes and virus-encoded proteins.

This phenomenon is particularly visible in Fig. Like GFLV, these viruses all induced modifications of the ER network but with noticeable differences, suggesting that these viruses use different mechanisms to recruit membranes.

Mock-inoculated protoplasts referred to as healthy protoplasts were used as a control. Panel J is a 2.

In this case, no immunolabeling corresponding to replication-specific proteins was found to localize with it any more.

The 2C protein was predominantly found in the perinuclear viral compartment Fig. This plant virus article is a stub.

Grapevine Fanleaf Virus Replication Occurs on Endoplasmic Reticulum-Derived Membranes

Finally, the CP-containing spikes in Fig. View publishing information about this page. Fanleaf and LeafrollWashington State University.


T-BY2 protoplasts were prepared from wt and transgenic cells from 4-day-old cultures.

It can therefore be concluded that both de novo phospholipid biosynthesis and ER-Golgi integrity are required for efficient GFLV replication. Sucrose gradient fractionation and analysis.

Brefeldin A inhibits cell-free. In view of the close resemblance between both systems, we suggest that GFLV could use a vurus mechanism to recruit membranes for replication purposes. Similar information is available with animal viruses and has been described extensively in the literature, more particularly for poliovirus, the type member of picornaviridae.

Grapevine Fanleaf Virus Replication Occurs on Endoplasmic Reticulum-Derived Membranes

Articles with ‘species’ microformats All stub articles. To demonstrate whether the ER-derived aggregates were involved in viral replication, double-labeling experiments were performed with anti-dsRNA and anti-VPg antibodies.

Four bands of VPg-containing precursors fnaleaf migrated in the gel with apparent molecular masses of ca. Effects of site-directed mutagenesis on the presumed catalytic triad and substrate-binding pocket of grapevine fanleaf nepovirus kDa proteinase. Retrieved from ” https: Immunocytochemical localization of TYMV-coded structural and non-structural proteins by the protein A-gold technique.

Cells were harvested at 48 h p. Image acquisition was performed as previously described When superimposed, dsRNA and VPg labeling rarely coincided strictly, as judged by the very low number of yellow spots Fig.

Similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest common ancestry of these virus families. Protoplast viability in the absence or presence of drugs was assessed 48 h after transfection by using FDA staining.

These spots proved to be localized at the tip of MP-labeled tubules Fig.

In spite of being derived from the same polyprotein as CP, the MP was rarely detected anywhere else than in tubules.


Enzyme cytochemical identification of single-stranded and double-stranded RNAs in virus-infected plant and insect cells. VPg Northern-immunoblots as a means for detection of viral RNAs in protoplasts or plants infected with grapevine fanleaf nepovirus.

Sequence analysis and coat protein cistron location”.

The membranes were then incubated with goat anti-rabbit IgG A or goat anti-mouse IgG B and C coupled to horseradish peroxidase and revealed by chemiluminescence. Browse related by Tag grapesgrapes disease management. For immunofluorescence microscopy, polyclonal anti-2B antibodies named anti-MP raised in rabbits as described in 53 were used at a 1: Grapevine fanleaf virus symptoms – stunted, zig-zag shoot with fan-shaped leaves. In some cases, the transmembrane domains responsible for their anchoring on specific membranes could be identified 183556 allowing the formation of dedicated structures where coupled translation and synthesis of both minus- and plus-strand RNA take place 8.

Formation of plant RNA virus replication complexes on membranes: To compare the distribution of the viral proteins involved in replication with that of proteins involved in movement and encapsidation, we analyzed the intracellular distribution of the movement protein 2B MP and the coat protein 2C CPboth of which are dispensable for replication Grapevine fanleaf degeneration disease has two distinct syndromes, or sets of symptoms, depending on the virus strain and host response to infection.

Viral proteins not involved in replication are not restricted to the virus-induced ER-derived compartment.